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KMID : 1059520070510010000
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Journal of the Korean Chemical Society
2007 Volume.51 No. 1 p.0 ~ p.0
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Detection of Hepatitis B Virus Using Micro-PCR and Real-Time PCR Methods
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Kang Won
Park Sang-Bum
Nam Youn-Hyoung
Ahn Young-Chang
Lee Sang-Hyun
Jang Won-Choeul
Park Su-Min
Kim Jong-Wan
Chong Song-Chun
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Abstract
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Hepatitis B is a serious public health problem leading to chronic infection and liver cancer. Quantitation
of circulating hepatitis B virus (HBV) is important for monitoring disease progression and for assessing the response to
antiviral therapy. In this study, by using Real-Time PCR and novel Micro-PCR assay method, we measured HBV concentration
in the clinical sample. A total of 120 serum samples from patients with HBV infection collected was in Dankook
university hospital to compare the detection limit, sensitivity, specificity and reproducibility of the two assay
methods. These findings of this study suggest that Micro-PCR and Real-Time PCR assay methods are comparable to each
other in there detection limit, sensitivity, and reproducibility for HBV DNA quantitation. However, Micro-PCR assay is
more efficient than Real-Time PCR method, because Real-Time PCR is not so time - consuming, technically easy and need
to reagent of a small quantity. It will be useful for rapid and reliable clinical diagnosis of HBV in many countries.
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KEYWORD
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Hepatitis B Virus, Real Time-PCR, Micro-PCR
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